By Rajni Hatti-Kaul
A mix of polymers, or one polymer and a salt, in an aqueous medium separates into stages: this phenomenon comes in handy in biotechn- ogy for product separations. Separation of organic molecules and debris in those aqueous two-phase structures (ATPS) used to be initiated over forty years in the past through P.-Å. Albertsson, and later proved to be of titanic software in biochemical and phone organic learn. a lift within the software of ATPS was once visible whilst difficulties of separations in biotechnology techniques have been encountered. Its simplicity, biocompatibility, and amenability to effortless scaleup operations make using ATPS very beautiful for large-scale bioseparations. regardless of the benefits ATPS enjoys over different separation ideas, the applying of two-phase platforms has for a very long time been constrained to chose labora- ries. fresh years have, notwithstanding, proven a pattern within which expanding numbers of researchers hire two-phase partitioning innovations in either simple and utilized learn.
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Additional info for Aqueous two-phase systems. Methods and protocols
Albertsson (1). Others have also been published (3,4,10). Some convenient two-phase systems are shown in Table 4. d. Selection of a volume ratio: As a rule of thumb, those who are using aqueous two-phase systems for the first time should choose equal top and bottom phases volumes to facilitate sampling and protein partition coefficient determination. e. Scale: For analytical purposes, 5 or 10 grams systems are very convenient. f. Buffer: The selection of the buffer should be based upon the desired pH range.
Louis, MO. CT. cDarmstadt, Germany. dWarrington, PA. eUppsala, Sweden. fDormagen, Germany. bDanbury, stock salt solution of the desired pH, are prepared. In the systems for affinity partitioning (Chapters 29–32), stocks of derivatized polymers are also prepared. ). The stock solutions may be stored before use (see Note 4). ) Charged polymer Comments Changes partition coefficient; used in crosspartitioning experiments Chages partition coefficient; used in crosspartitioning experiments Bactericide Bactericide Changes partition coefficient of target protein Changes partition coefficient and phase equilibrium 1.
Technol. 11, 730–735. Preparation of ATPS 23 3 Preparation of Aqueous Two-Phase Systems Daniel Forciniti 1. Introduction The most common aqueous two-phase systems (ATPS) are created using either two incompatible polymers or one polymer and a salt (1). These mixtures separate into two liquid phases which are in equilibrium; each phase is enriched in one or the other of the respective phase forming components. Both the phases have a water content of about 85–90%. The most frequently made systems have been those of polyethyleneglycol/dextran (PEG/Dx) and PEG/ phosphate.
Aqueous two-phase systems. Methods and protocols by Rajni Hatti-Kaul