By John R. W. Masters
This re-creation of Animal mobilephone tradition covers new or up to date chapters on cellphone authentication, serum-free tradition, apoptosis assays, FISH, genetic amendment, scale-up, stem telephone assays, three-d tradition, tissue engineering and cytotoxicity assays. certain protocols for a large choice of equipment give you the middle of every bankruptcy, making new method simply obtainable. every body operating in organic and clinical learn, even if in academia or a advertisement association, practicing mobilephone tradition will gain tremendously from this e-book.
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Additional info for Animal Cell Culture: A Practical Approach
25%), and rolling. Yields will be very similar to those obtained in flasks, assuming enough medium was added. This method has the advantage of allowing the medium volume/surface area ratio to be altered easily. Thus after a growth phase the medium volume can be reduced to get a higher product concentration. 2 Step 2: roller bottle modifications The roller bottle system is still a multiple process, and thus inefficient in terms of staff resources and materials. To increase the surface area within the volume of a roller bottle, the following vessels have been developed (see also Figure 3).
G. g. high voltage discharge, UV light, high energy electron bombardment). One or more of these methods are used by manufacturers of tissue culture grade plastics. The result is to increase the net negative charge of the surface (for example by forming negative carboxyl groups) for electrostatic attachment. Surfaces may also be coated to make them suitable for cell attachment. A tissue culture grade of collagen can be purchased which saves a tedious preparation procedure using rat tails. The usefulness of collagen as a growth surface is also demonstrated by the availability of collagen-coated microcarrier beads (Cytodex-3, Pharmacia).
The most common additives are insulin (5 mg/litre), transferrin (5-35 mg/litre), ethanolamine (20 uM), and selenium (5 ug/litre) (5). Cell aggregation is often a problem in suspension cultures. 1). This problem has also been overcome by including very low levels of trypsin in the medium (2 ug/ml). 0 or lower. 8 is usually inhibitory to cell growth. Factors affecting the pH stability of the medium are buffer capacity and type, headspace, and glucose concentration. The normal buffer system in tissue culture media is the carbon dioxide bicarbonate system analogous to that in blood.
Animal Cell Culture: A Practical Approach by John R. W. Masters